Composite

Part:BBa_K3693008:Design

Designed by: Jie Gao   Group: iGEM20_BJ101HS   (2020-10-22)


Purification of LTF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
    Illegal BglII site found at 859
    Illegal BglII site found at 1414
    Illegal BglII site found at 1585
    Illegal BglII site found at 1846
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 309
    Illegal BsaI.rc site found at 483
    Illegal SapI.rc site found at 1109


Design Notes

We add his-tag at both N-terminal and C-terminal for affinity purification


Source

The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.

References